Fig 1: mRNA and mature Dynorphin B expression in differentiated and undifferentiated SH-SY5Y following scAAV-pDyn transduction. SH-SY5Y cells were transduced with scAAV-pDyn at three different MOIs (5,000, 1,500, and 500) and harvested 96 h post-transduction. pDyn mRNA expression (A) and Dyn B levels in the medium (B) and in the cell extract (C) were analyzed in parallel from each infection well (n = 5) of differentiated and undifferentiated SH-SY5Y cells. (A) mRNA expression of pDyn was normalized to GAPDH in differentiated cells () and undifferentiated cells (). (B) Amount of DynB was quantified per well in the medium of differentiated cells () and undifferentiated cells (). (C) Amount of DynB was quantified per well in the cell extract of differentiated cells () and undifferentiated cells (). Data show five biological replicates with mean ± SD (****P-value ≤ 0.0001; ***P-value ≤ 0.001; **P-value ≤ 0.01; *P-value ≤ 0.05; ns—non-significant following a two-way ANOVA).